(C)Katsuya Obara

Researchers at University of Tsukuba, have developed a method for culturing fertilized chick eggs without their shells. The eggs were placed in an artificial culture vessel made of transparent film, allowing for real-time observation of the chick embryo's development from laying to hatching.

Tsukuba, Japan—Efforts to observe chicken embryonic development date back to Aristotle date back to 300 BC. However, because the chicken's eggshell is opaque, it has been necessary to break the shell to observe the embryonic development occurring inside the egg. As a result, attempts have been made to develop transparent artificial culture vessels. A previously reported method involves removing embryos from their shells after being incubating them for 3 days and then hatching them in a transparent artificial culture vessel. However, when fertilized chick embryos immediately after laying (0-day-old embryos) were cultured using this method, they did not develop normally.

The researchers investigated the cause of this issue and discovered that the yolk membrane covering the surface of the blastoderm (the area where local cell division occurs during egg development) became dry by the third day of culture (3-day-old embryos). To prevent the yolk membrane from drying out, the researchers utilized a rotary shaker with the top plate rotating at an angle of 7°. This innovation prevented the membrane covering the blastoderm from drying out, which subsequently improved the survival rate of the 3-day-old embryos. Furthermore, as these embryos continued to be cultured using the conventional shell-less method, embryonic development progressed, leading to the successful hatching of several normal chicks on the 21st day after the start of incubation.

The results of this research are expected to provide a foundation for various applications, including chick embryo development, toxicity assessments, and regenerative medicine.

Original Paper

Title of original paper:

Real-time visualisation of developing chick embryos cultured in transparent plastic films from the blastoderm stage until hatching

Journal:

Scientific Reports

DOI:

10.1038/s41598-024-72004-y

Correspondence

Professor Emeritus TAJIMA Atsushi
Institute of Life and Environmental Sciences, University of Tsukuba

Dr. OBARA Katsuya
Doctoral Programs in Life and Agricultural Sciences, Degree Programs in Life and Earth Sciences, Graduate School of Science and Technology, University of Tsukuba (Current position: Visiting Researcher of the Institute of Life and Environmental Sciences, University of Tsukuba)

Assistant Professor OBARA (HENMI) Chizuka
Faculty of Veterinary Medicine, Imabari Campus, Okayama University of Science

Related Link

Institute of Life and Environmental Sciences