Researchers at the University of Tsukuba have rediscovered and successfully cultivating Rhabdamoeba marina—a rare marine amoeba that has only been reported in two cases in the past century. Using this culture strain, they performed a comprehensive analysis of its genetic sequence, revealing for the first time the phylogenetic position of this enigmatic amoeba, and proposed a novel taxonomic classification based on their research findings.

Tsukuba, Japan—Rhabdamoeba marina (R. marina) is a small marine amoeba, first discovered and described in England in 1921. The amoeboid cells of R. marina, characterized by their near immobility, can produce flagellated cells with two rearward-extending flagella through budding under conditions of prey scarcity. Despite these distinct characteristics, the taxonomic classification of R. marina has remained unverified because only two cases have been documented, including the original description. Researchers successfully established a culture strain of R. marina from seawater sourced from the coast of Tottori Prefecture, Japan. Analyzing the genetic sequence of this strain, they found that R. marina does not align with its previously assumed taxonomic group but is closely related to chlorarachnid algae within the phylum Cercozoa. Therefore, the researchers have advocated for the reclassification of R. marina into the class Chlorarachnea.

Through this study, the gene sequence of R. marina—a scarcely encountered and rare amoeba--has been unveiled for the first time, and its phylogenetic position has been clarified. The study highlights the importance of observing environmental samples for the rediscovery of unicellular organisms such as R. marina that lack genetic data. Such efforts are indispensable for understanding microbial diversity.

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This work was supported by JSPS KAKENHI, Grant Numbers 19H03282 and 18J02091.

Original Paper

Title of original paper:

Rhabdamoeba marina is a heterotrophic relative of chlorarachnid algae

Journal:

The Journal of Eukaryotic Microbiology

DOI:

10.1111/jeu.13010

Correspondence

Assistant Professor SHIRATORI Takashi
Institute of Life and Environmental Sciences, University of Tsukuba

Related Link

Institute of Life and Environmental Sciences